MONDAY, JUNE 19, 12:45 – 13:45


Club A

Discover the Difference - New efficient LC and LC/MS Solutions for any Application and Budget
Michael Frank, Jan Lembcke and Martin Vollmer– Agilent Technologies

From routine analysis to cutting-edge research, the Agilent InfinityLab LC Series provides the broadest portfolio of LC systems and solutions for any application and budget to achieve highest efficiency. Combined with industry-leading mass spectrometry performance as well as InfinityLab column technology, Agilent demonstrates the benefits of new innovative LC and LC/MS solutions for a wide range of applications. Learn how state-of-the-art OpenLAB Software and CrossLab Services take your operations to the next level of efficiency.


Club B

Modernizing, Managing, and Complying to Regulatory Advances in Science
E. Nigel

This tutorial covers the strategy and business impacts of transferring liquid chromatography methods to new technologies, between laboratories, and even between companies when necessary. Specifically: what are the ‘rules’ that companies/laboratories must follow, how are they changing, and what does it mean? Waters will demonstrate solutions that have been developed to efficiently transfer methods while maintaining compliance in regulated laboratories. Throughout this presentation we will be using examples from actual situations, and discussing real-world troubleshooting tips to consider when difficulties are encountered. The process required to modernize and manage method transfers is often complex, and may benefit from an on-site consultation by Waters Professional Services in order to provide a tailored solution for your specific quality system.


Club C

Please follow the link for registration:

Intact Protein Analysis of Biopharmaceuticals
K. Cook, J. Bones

Biopharmaceuticals represent over $150 Billion in revenue globally each year and continues to grow. Alongside this there is the requirement for the comprehensive characterisation of biopharmaceuticals during all stages of drug development and manufacture to ensure safety and efficacy. Liquid chromatography with mass spectrometry (LC-MS) allows the characterisation of intact and digested biopharmaceuticals, typically antibodies, to determine glycosylation patterns, charge variants, antibody-drug conjugate ratio determination and more. In the seminar, we will present a comprehensive overview of the latest developments in liquid chromatography separations for the characterization of bio therapeutic proteins, including new chemistries and UHPLC enhancements to provide faster, more reproducible and information-rich workflows. The use of a single UHPLC/ high resolution Orbitrap mass spectrometer platform to measure multiple critical attributes including Glycoforms, PTM’s, intact mass and sequence conformation will also be discussed and demonstrated.


Club D

Wide pore monolithic silica of various functionalization: Protein A, C18, C8 and C4, in high performance liquid chromatography for large molecule separations
E. Machtejevas

In the last years the pharmaceutical market has changed dramatically from small molecules to protein-based drugs. The development of biological entities is strong increasing because biotechnological processes now enable a production with reasonable costs. That implies a demand of suitable analytical methods for process monitoring and quality control of biomolecules with therapeutic purposes. Especially the HPLC is the mostly used analysis method. Most important for the HPLC analysis are the properties of the column. For bigger peptides, proteins or antibodies a new type of columns is needed which provide a good permeability, better mass transfer and a better selectivity. In contrast to conventional packed-particle columns, wide pore (300 A) monolithic silica columns are made of a single continuous-bed rod of high purity porous silica that is then bonded with C18, C8, CN, Protein A. Monolithic columns remove backpressure as the primary consideration in method development and give back the flexibility of choices in flow rates for much higher throughput, column lengths for superior resolution, and solvent choices for optimum selectivities that smaller and smaller sized packed-particle columns have slowly taken away over the decades. Because they have no individual particles to shift or break, column performance is very consistent over much longer lifetimes, making them ideal for relatively ”dirty/matrix rich” sample analysis. Their high permeability also makes them very forgiving of shortcuts and timesaving in sample preparation as well as easier to aggressively flush out to reequilibrate. This presentation will guide you through the world of wide pore monolithic silica materials. Benefits will be demonstrated with many application examples including pharmaceutical and bioanalysis separations (proteins, antibodies, etc.), calibration curves, recovery calculations, and method robustness overviews.

Powerful analytical approaches combined: An introduction to TLC-MS
A. Michalski

Thin layer chromatography is a classic in analytical chemistry. No other chromatographic method offers the same level of transparency and flexibility in terms of chemistries used, and options to combine solvent systems for separation. In recent years, the separation efficiency of TLC was significantly enhanced by the introduction of smaller particles sizes and thinner layers. Here, we demonstrate how these developments integrate into a mass spectrometry-based workflow that complements current HPLC-MS workflows in drug synthesis or quality control labs.


Club E

Is the supercritical fluid extraction/chromatography promising for metabolite identification?
A. Demailly

Despite the fact that SFC and SFE are established techniques in analytical chemistry, their use in bioanalysis and particularly in metabolite identification are limited. In here we show some examples regarding the application of SFE-SFC for analysis of spiked biological matrices samples used in metabolism profiling such as, plasma, blood, urine and feces. The easiness of sample preparation and the full automation of sample extraction prior to the separation could possibly give SFE-SFC technology an advantage in the field of metabolite profiling.

What is the advantage of SFC/MS?
Y. Fujito

SFC/MS has a great potential in high-throughput and simultaneous analysis of a wide variety of compounds and has been widely used in recent years. We will discuss advantages and benefits of SFC/MS in multiresidue analysis through comparison with LC/MS. Moreover, we will also show a basic concept for optimization of the MS parameters in SFC/MS analysis because SFC/MS requires a specific optimization of MS parameters for maximizing sensitivity due to the difference of the mobile phase conditions from those of LC/MS.

Teaching Practical HPLC at a Distance
N. Turner

Teaching practical and analytical techniques to large numbers of science undergraduate students has always been an issue; doubly so, if you are a distance-learning institute. It has always being difficult for students to gain hands-on experience with complex liquid chromatography, despite this experience been needed to support their theoretical studies. The ability to use such equipment is important for graduatesd in the workplace. The Open University is the UK's largest academic institution and a world pioneer in distance learning. To date, the OU has taught over two million students and we are applying this experience in developing online, accessible resources to allow practical studies to be achieved at a distance, through the OpenSTEM Labs. In this talk Dr Turner will present how the Open University, in partnership with Shimadzu, are developing a student friendly, remote access UHPLC platform which will enable us to teach students practical HPLC, from first principles, through to the practice of method development. -- The Open University is incorporated by Royal Charter (RC 000391), an exempt charity in England & Wales and a charity registered in Scotland (SC 038302). The Open University is authorised and regulated by the Financial Conduct Authority.

TUESDAY, JUNE 20, 12:45 – 13:45


Club A

How to manage 10.001 Prep-LC Samples with a small analytical Team?  -  New Pathways with exceptional Accuracy and Flexibility
Colinda van Tilburg, Mercachem

We share our approach to handle a lot of samples but don’t forget that these samples were made with lots of love and effort. Some chemists made libraries with large number of samples, some make one compound. Both want to get a pure compound back with the highest recovery possible. We have found a workflow to manage this reliable, day after day.

Stefan Ullrich and Ronald Guilliet – Agilent Technologies

Agilent offers high-efficiency InfinityLab LC and LC/MSD solutions ranging from analytical scale up to preparative scale for purification of multiple grams. A portfolio of state-of-the-art LC purification instruments offers you scalable solutions to grow with your needs. Don’t miss a single compound through a wide range of fraction collectors and detectors including mass-selective detection.


Club B

Combining the Powerful Particles with Powerful Selectivities
J. Anspach

The modern age of chromatography has seen inventions such as Sub 2 µm materials and Core-Shell particles that provide the scientist with higher resolving power through higher efficiencies. If one, however, looks at the resolution equation, resolution is significantly more dependent on selectivity than on theoretical plates. Therefore, in order to take full advantage of the extra efficiency that the newer generation particle provide, we need modern, improved stationary phase selectivities to work with the high efficiency platforms. In this talk we will show how modern surface chemistry technologies in conjunction with modern UHPLC particle technologies provide significant separation advantages in difficult applications.

Chromatographic Behavior of Biomolecules when using Controlled Surface Charge (U)HPLC Sorbents
A.C. Sanchez

Recent developments in (U)HPLC sorbents include materials where the surface charge has been intentionally modified and controlled. The utility of sorbents with a controlled, slightly positive surface charge has been shown to improve the chromatographic performance of basic, small molecules when using common, low ionic strength mobile phases [1,2]. However, the use of these new, controlled surface charge sorbents for the analysis of intact biomolecules has not been significantly explored. In this contribution, the effect of sorbent surface charge on the chromatographic behavior of a variety of biomolecules will be explored. Biomolecule pI and amino acid composition will be carefully chosen to span ranges of practical interest. The interplay between biomolecule properties and sorbent surface charge will be explored.


Club C

Sciex  Lunchtime Vendor Seminar
Gavin Fisher, Sr. Director Liquid Chromatography (LC) Business, Sciex
Erika Lin, Product Manager - Micro Nano LC, Sciex
Steve Lock, Market Development Manager, Sciex

Scientists working with Electrospray- Mass Spectrometry (ESI-MS) must choose the best separation technique and flow regime for their application. This choice is driven by many factors including analyte type, Limits of detections, sample matrix and quantity of sample. In this workshop, we will take the example of peptide quantitation to discuss two alternatives to traditional analytical flow ESI-MS for peptide quantitation: micro-flow LC-MS and CESI-MS.

When the sample quantity is limited and extra sensitivity is needed, micro-flow LC-MS can increase sensitivity by up to 5 x, compared to analytical flow LC-MS. This approach provides accurate and reproducible results for applications such as peptide quantitation and is ideal for high throughput trap-elute workflows. The sample is loaded at a high flow onto a trap column and then back flushed through a LC column at micro flow rates to the MS detector. The result is up to a 5x shorter run time compared with direct injection micro flow LC-MS.

For charged and polar analytes in minute sample volumes, intact proteins and challenging peptides CESI-MS can be the right choice.  For example, highly basic peptides are difficult to analyze by LC/MS methods as they bind to columns and LC systems and have very poor chromatographic properties.  We will describe the development of a CESI-MS method for the analysis of highly basic neuropeptides at physiological levels in biological fluids, not possible with LC/MS.


Club D

Innovation in Enantiomeric Separations and Related Fields
P. Franco

Over the last four decades there has been a significant growth in the acceptance and implementation of enantiomer separations in the life science industries. DAICEL has been the principal company in this area by developing new innovative products and supporting new users with local technical support. The list of recent new product introductions and supporting activities include commercial introduction of Immobilized polysaccharide-derived Chiral Stationary phases (CSPs) in 2004, the development of innovative screening strategies and as a result, the launch of more than 750 products are some of the achievements in the quest for solutions to customer needs. This continual innovation is inspired by the unmet needs of the scientific community. In the last 12 months this has included the design of new chiral selectors with complementary and enhanced selectivities (CHIRALPAK IG and IB-N); the search for faster and more efficient resolutions (sub 2 µm based CSPs) and extending our experience into the quest for enhanced selectivities in achiral separations (DCPack SFC A and SFC B). This presentation will review the major historical innovations, the recently introduced products and the future activities of Daicel and Chiral Technologies.


Club E

Please follow the link for registration:

Enhancements for Improved Routine Impurity Analysis
R. Bauder

The presence of a known impurity or an unknown contaminant, even at trace levels, can have serious consequences for the safety or quality of a product or the environment, be that a pharmaceutical, food and beverage or a water/soil sample. Especially within routine and manufacturing supporting laboratories, false positives or false negatives can have significant quality impacts as well as financial consequences (i.e. loss of production batch). A range of examples will be presented, which not only provides increased quality of the assay results itself, but also employs state-of-the art trouble shooting strategies and multi-detection concepts. This seminar will showcase our comprehensive new workflow solution for impurity analysis with a focus on pharmaceutical impurities as well as environmental testing and how quality can be assured at each stage of the workflow. We shall reveal new technology to bring mass spectrometry to chromatographers and how the Thermo Scientific™ Chromeleon™ CDS software can be used for both HPLC and IC separations and mass spectrometry, whilst ensuring data integrity and reproducible performance. Furthermore, we shall offer alternative liquid chromatography detection methodologies for difficult to detect compounds using charged aerosol detection and suppressed conductivity detection.

WEDNESDAY, JUNE 21, 12:45 – 13:45


Club B

New Developments and Applications for 2D-LC – New expanding Capabilities
Matthias Pursch - Dow Deutschland Anlagen GmbH & OHG

Multidimensional LC is becoming a key technology for complex sample analysis in chemical, pharma and biomedical areas. This presentation highlights several chemical applications using comprehensive, heart-cutting and high-resolution sampling 2D LC. Results for target component analysis and general sample characterization will be presented. We describe the benefits of active solvent modulation (ASM) as new technology for 2D sensitivity enhancement when strong 1D effluents (methanol, acetonitrile, THF) are used. Successful examples using ASM will be shown for SEC and HILIC methods in 1D followed by RPLC separations in 2D.

Jens Trafkowski and Ulrich Eberhardinger  – Agilent Technologies

A variety of 2D-LC concepts have been established and scientists have successfully proven the benefits in different application fields. We share the latest technological developments and real-life examples, how the most difficult chromatographic tasks are solved in a world of complex samples from research to manufacturing.


Club C

Please follow the link for registration:

Next Generation Innovations in High Performance LC/MS – Bruker Daltonics

Discover new ways to apply mass spectrometry to today’s most pressing analytical challenges. Innovations such as Trapped Ion Mobility (TIMS), eXtreme Resolution MRMS technology for Isotopic Fine Structure (IFS) signatures and leading software suites for metabolomics, screening, protein characterization and imaging are pushing scientific exploration to new heights. Our mass spectrometry solutions enable scientists to make breakthrough discoveries and gain deeper insights.
Learn more about the new “Elute” UHPLC and nanoLC systems from Bruker with maximum flexibility in configurations tailored for demanding MS applications and unsurpassed performance in precision, reproducibility and RT stability

Recent Innovations at Bruker
Dr. Michal Bohac

Next Generation Ion Mobility Spectrometry: the novel timsTOF IMS-QTOF
Dr. Andreas Jakob

MetaboScape – Bridging the Mass Spec Data with Biological Knowledge in Metabolomics
Dr. Arnd Ingendoh


Club D

Reduced Time to market by Data Modeling in Analytics of Biopharmaceuticals
I. Molnár

The vendor seminar addresses the need for faster drug development for better efficiency of product life cycle and plant utilization. It shows ways of characterizing new drugs, such as Monoclonal Antibody-Drug conjugates (ADC’s) by demonstrating how the newest generation of HPLC modeling software vastly reduces development time and improves communication between applicants for new drugs and the regulatory authorities. The seminar emphasizes problems and opportunities of long development times for new drugs and wasted value of drugs due to OoS methods not fit for purpose, slow communication between the industry and regulatory bodies. It points out how HPLC modeling software speeds up drug development and points out how a software based knowledge management documentation facilitates communications about QC issues.


Club E

Successful Development and Implementation of Peptide Mapping and SEC-Based Protein Aggregate Determinations for Biotherapeutic Characterization
P. Jayaraman, S. Koza

Peptide mapping and size-exclusion chromatography (SEC) are frequently used methods during the development and commercialization of biotherapeutics. Using relevant data, this technical seminar will review important principles and practices to achieve high-quality separations using columns specifically designed and QC tested to deliver reproducible results in validated methods.

Symposium secretariat: C-IN, 5. kvetna 65, 140 21 Prague 4, Czech Republic | tel.: +420 261 174 305
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